Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: (A) Representative images showing GFP and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, AAV-DIO-BDNF-mCherry+sgRNA, AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing, Injection, Virus

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression pattern in lateral septum (A), preoptic area (C), PAG (E), periLC (G). scale bar=250 μm. The pictures in the bottom row of each panel represent the magnified pictures shown in the boxed area in each column. Number of cFos positive cells in in lateral septum (B), preoptic area (D), PAG (F, periLC (H). n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing

Journal: bioRxiv

Article Title: Identification of a neural basis for anorexia nervosa

doi: 10.64898/2026.02.07.704578

Figure Lengend Snippet: Representative images showing mCherry (red) and cFos (cyan, arrows) expression pattern in VH in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice (related to ). Scale bar=200 μm.

Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

Techniques: Expressing, Injection

Journal: Cells

Article Title: Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons

doi: 10.3390/cells9030644

Figure Lengend Snippet: Double immunofluorescent staining against neurotrophins and their receptors. ( A , B ) Immunofluorescent staining against P75/NGFR (red color) and NGF (green color). ( C , D ) Immunofluorescent staining against TRKA (red color) and NGF (green color). ( E , F ) Immunofluorescent staining against TRKB (red color) and BDNF (green color). Blue color: DAPI. Yellow arrowheads ( B , D ) and white arrowheads ( F ) indicate, respectively, NGF expressing- and BDNF expressing-cells within the ameloblastoma epithelium. Abbreviations: BNDF, Brain Derived Neurotrophic Factor; ep, epithelium; NGF, Nerve Growth Factor; st, stroma. Scale bars: ( A , C , E ): 400 μm; ( B , D ): 100 μm; ( F ): 50 μm.

Article Snippet: The following primary antibodies were used: Goat anti-NGF (1:100, AF256NA, R&D Systems, Minneapolis, MN, USA), Rabbit anti-BDNF (1:100, ab108319-100UL, Abcam, Cambridge, UK), mouse IgG1 α-neurofilament (α-NF) antibody (1:100, Hybridoma Bank, Iowa City, IA, USA), rabbit mAb anti-neurofilament (1:200, Cell Signaling Technology, 2837), mouse mAb anti-Vimentin (1:100, DAKO, M0725), rabbit mAb anti-TrkA (1:50, Abcam, ab76291), rabbit pAb anti-TrkA (1:100, Sigma-Aldrich, 06-574), rabbit pAb anti-TrkB (1:100, R&D Systems, AF1494), rabbit pAb anti-TrkC (1:100, R&D Systems, AF1404), rabbit mAb anti-nerve growth factor receptor (NGFR/p75; 1:100, Sigma-Aldrich, N3908), rabbit pAb anti-β-III-Tubulin (1:200, Abcam, ab18207), rabbit pAb anti-Synapsin I (1:100, Abcam, ab64581), rabbit mAb anti-wide spectrum Cytokeratins (1:100, Abcam, ab9377), rabbit pAb anti-Notch1, Notch2, Notch3 [ ], rabbit pAb anti-Dll1 (1:100, ab1054, Abcam), rabbit pAb anti-Dll4 (1:50, ab7280, Abcam), and rabbit pAb anti-Jag1 (1:50, ab7771, Abcam).

Techniques: Staining, Expressing, Derivative Assay

Journal: The Journal of Neuroscience

Article Title: Elevated TRPV4 Levels Contribute to Endothelial Damage and Scarring in Experimental Spinal Cord Injury

doi: 10.1523/JNEUROSCI.2035-19.2020

Figure Lengend Snippet: Primary antibody information used for immunohistochemistry analysis

Article Snippet: The fluorescence IMV was plotted as mean ± SEM in GraphPad Prism (version 5.01, GraphPad Software). table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Primary antibody Dilution Producer company Code number Anti-TRPV4 1:500 Alomone Labs, Israel ACC-034 Anti-RECA-1 1:100 Abcam, UK ab22492 Anti-NF 1:1000 Abcam, UK ab8135 Anti-Iba-1 1:200 FUJIFILM Wako, Japan 019-19741 Anti-laminin 1:50 Sigma L9393 Anti-GFAP 1:500 Abcam, UK ab10062 Anti-ZO-1 1:50 Invitrogen 40-2300 Anti-occludin 1:50 Invitrogen 33-1500 Anti-collagen-IV 1:500 Abcam, UK ab6586 Anti-nitrotyrosine 1:100 Abcam, UK ab183391 Anti-CD-68 1:200 Abcam, UK ab955 Anti-TGF-β1 1:100 Abcam, UK ab25121 Anti-CD-206 1:500 Abcam, UK ab64693 Anti-BDNF 1:300 Alomone Labs, Israel AGP-021 Anti-NT-3 1:200 Alomone Labs, Israel ANT-003 Anti-vWF 1:50 Millipore AB7356 Anti-ANG-1 1:200 Abcam, UK ab133425 Anti-NG-2 1:500 Abcam, UK ab129051 Anti-α-SMA 1:200 Abcam, UK ab21027 Anti-connexin-43 1:500 Abcam, UK ab11369 Anti-Tuj-1 1:1000 Abcam, UK ab18207 Anti-growth cone 1:3 Abcam, UK ab7762 Anti-NeuN 1:1000 Abcam, UK ab177487 Open in a separate window Primary antibody information used for immunohistochemistry analysis table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Secondary antibody Dilution Producer company Code number Goat anti-rabbit AlexaFluor-488 1:200 Abcam, UK ab150077 Goat anti-rabbit AlexaFluor-568 1:200 Abcam, UK ab175471 Goat anti-rabbit AlexaFluor-647 1:200 Abcam, UK ab150083 Goat anti-mouse AlexaFluor-568 1:200 Abcam, UK ab175473 Goat anti-mouse AlexaFluor-488 1:200 Abcam, UK ab150117 Goat anti-guinea pig AlexaFluor-488 1:200 Abcam, UK ab150185 Chicken-anti-goat 647 1:200 Invitrogen, USA A-21469 Open in a separate window Secondary antibody information used for immunohistochemistry analysis Immunocytochemistry For immunofluorescence microscopy, third passage hCMEC/D3 BBB cell line and HUVECs (0.5 × 10 5 cells/well) were cultured on a sterile rat tail collagen-I (Sigma Millipore)-coated coverslip in 24-well plates.

Techniques: Immunohistochemistry